Modifying Enzymes
New England Biolabs, Inc. cAMP-dependent Protein Kinase (PKA), catalytic subunit – 100000 units
The catalytic subunit of cAMP-dependent Protein Kinase (PKA) is a serine/threonine protein kinase. The recognition motif for phosphorylation by PKA is RRXS/TY, where Y tends to be a hydrophobic residue.
Sigma Aldrich Fine Chemicals Biosciences Lysozyme from chicken egg white BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein | 12650-88-3 | MFCD00131557 | 1g
Lysozyme from chicken egg white BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein | 12650-88-3 | MFCD00131557 | 1g
Sigma Aldrich Fine Chemicals Biosciences Amidase from Pseudomonas aeruginosa recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase >=200 units/mg protein (biuret) | 9012-56-0 | MFCD00166319 | 1KU
Amidase from Pseudomonas aeruginosa recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase >=200 units/mg protein (biuret) | 9012-56-0 | MFCD00166319 | 1KU
ABclonal Technology T4 RNA Ligase 1
T4 RNA ligase 1 catalyzes the formation of a 3 to 5 phosphodiester bond by joining the 5 phosphate end of a nucleotide to the 4 hydroxyl end, accompanied by the hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA, DNA, and dinucleoside pyrophosphates.
Worthington Biochemical Corporation DNA'ASE 5MG
Deoxyribonuclease I, Chromatographically Purified, Equivalent to 1X Crystallized Dnase, A Lyophilized Powder with Glycine as Stabilizer, Store at 2 deg. to 8 deg.C, Protect from Moisture, 5mg
Worthington Biochemical Corporation ELASTASE 25 MG
Elastase, Suspension, Code: ES, 2X Crystallized, Supplied as an aqueous suspension, Suitable for the Isolation of Type II lung cells, Store at 2 deg. to 8 deg.C, The diluted enzyme should be 0.22u filtered before use, 25mg
Worthington Biochemical Corporation PDS KIT 5/PK
Papain Dissociation System, Papain Vial, Code: PAP2, Vial reconstituted with 5mL of EBSS or equivalent yields a solution at 20U of papain permL in 1mm L-cysteine with 0.5mm EDTA, Store at 2 deg. to 8 deg.C, 5Vi
Biotium Topoisomerase (DNA) I, Mitochondrial (TOP1MT)(TOP1MT/568), CF740 conjugate, 0.1mg/mL
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can giv
Worthington Biochemical Corporation Protease, S. aureus, Sequencing Grade, STSEQ, 5x10ug
Protease, S. aureus, Sequencing Grade, STSEQ, 5x10ug. Chromatographically purified according to Drapeau, G, et.al, JBC, 247, 6720 (1972). Supplied as a lyophilized powder. Store at 2-8°C.
GENOVIS INC FabRICATOR LE, 5000 Units
FabRICATOR (IdeS) is a unique enzyme that digests IgG at a specific site below the hinge region, generating a homogenous pool of F(ab')2 and Fc/2 fragments. No other substrate besides IgG is known.The IgG is digested within 30 minutes, and there is no risk of overdigestion if the incubation time is prolonged. Since FabRICATOR digests IgG under physiological reaction conditions, the immunoreactivity is preserved. FabRICATOR digests all subclasses of human, and some classes of monkey, rabbit and sheep IgG. It has limited activity on mouse IgG2a and IgG3 - for digestion of these antibody species, we recommend using FabRICATOR Z .
Cayman Chemical Oleoyl-L-carnItInchlorIde 25mg
A long-chain acylcarnitine and GlyT2 inhibitor (IC50 = 340 nM); selective for GlyT2 over GlyT1 (IC50 = >10,000 nM); plasma levels are increased in patients with chronic kidney disease or end-stage renal disease receiving incident hemodialysis and these are associated with cardiovascular mortality