Modifying Enzymes
INTACT GENOMICS INC RNase Inhibitor, Murine 20,000 Units
RNase inhibitors are commonly used to inhibit and control RNase contaminants in enzymatic manipulations of RNA. Intact Genomics RNase Inhibitor is a 49.8 KDa recombinant protein of murine origin which specifically inhibits RNases A, B and C by binding noncovalently in a 1:1 ratio with high affinity. Moreover, it does not inhibit polymerase activity when used with Taq DNA Polymerase, Reverse Transcriptase. This Inhibitor from Murine is resistance to oxidation compared to the human/porcine inhibitors as it lacks the pair of cysteines found in the human/porcine versions. In addition, it is stable at less than 1 mM DTT concentration.Applications cDNA synthesis RT-PCR/RT-qPCR In vitro transcription/translation RNA labeling
Sigma Aldrich Fine Chemicals Biosciences Isovaleryl coenzyme A lith10MG
Isovaleryl coenzyme A an intermediate of the leucine catabolism. It is synthesized from -methylbutyric acid by the action of enzyme acyl CoA synthetase. The conversion of isovaleryl-CoA to methylcrotonyl-CoA is catalyzed by the enzyme isovaleryl-CoA dehydrogenase in leucine catabolism pathway. The enzyme isovaleryl-CoA oxidase also calalyzes this conversion in fatty acid -oxidation pathway.
Sigma Aldrich Fine Chemicals Biosciences Heparinase I and III Blend
Heparinase is an inducible non-extracellular heparin-degrading enzyme. Three types of heparinises are produced by flavobacterium heparinum and contains specific sequences of heparin.
Sigma Aldrich Fine Chemicals Biosciences Lipase from human pancreas
Pancreatic lipase fulfills a key function in dietary fat absorption by hydrolyzing triglycerides into diglycerides and subsequently into monoglycerides and free fatty acids.
New England Biolabs, Inc. Bst DNA Polymerase, Large Fragment – 8000 units
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5' to 3' polymerase activity, but lacks 5' to 3' exonuclease activity.
New England Biolabs, Inc. α-N-Acetylgalactosaminidase - 3000 units
a-N-Acetylgalactosaminidase is an exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing a-N-Acetylgalactosamine residues from oligosaccharides and N-glycans attached to proteins.
New England Biolabs, Inc. Exonuclease III (E.coli) – 5000 units
Catalyzes the stepwise removal of mononucleotides from 3'-hydroxyl termini of duplex DNA. A limited number of nucleotides are removed during each binding event, resulting in coordinated progressive deletions within the population of DNA molecules. The preferred substrates are blunt or recessed 3'-termini, although the enzyme also acts at nicks in duplex DNA to produce single-strand gaps. 3'-protruding termini are resistant to cleavage; the degree of resistance depends on the length of the extension,with extensions 4 bases or longer being essentially resistant to cleavage. Exonuclease III activity depends partially on helical structure and displays sequence dependence (C>A=T>G). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications. Exonuclease III has also been reported to have RNase H, 3'-phosphatase and AP-endonuclease activities.
Key Scientific Products DROP-IT CATALASE 3%, 5 per Pack
A Catalase test is useful for the identification of many different bacteria. Since Streptococci lack the enzyme to catalyze hydrogen peroxide, and all Staphylococci possess it, 3% Catalase is used to separate the species. Also, Listeria monocytogenes, which is catalase positive can be differentiated from beta-hemolytic Streptococci. Most Neisseria species are catalase positive. 15% Catalase is used for anaerobes, with most anaerobes being catalase negative with the exceptions of several species in the B. fragilis group and a few of the anaerobic cocci. 30% Catalase is used with acid fast bacilli.
New England Biolabs, Inc. Uracil-DNA Glycosylase (UDG) – 1000 units
E. coli Uracil-DNA Glycosylase (UDG) catalyses the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases).
ELASTIN PRODUCTS COMPANY INC HU SPUTUM LEUCOCYTE ELAST 1MG
Human Sputum Leucocyte Enzyme elastase 1mg
New England Biolabs, Inc. Mung Bean Nuclease – 7500 units
Mung Bean Nuclease is a single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions leaving blunt, ligatable ends.
New England Biolabs, Inc. α1-6 Mannosidase - 800 units
a1-6 Mannosidase is a highly specific exoglycosidase that removes unbranched a1-6 linked mannose residues from oligosaccharides. When used in conjunction with a1-2,3 Mannosidase, the a1-6 Mannosidase will cleave a1-6 mannose residues from branched carbohydrate substrates.
New England Biolabs, Inc. AluI Methyltransferase – 100 units
AluI Methyltransferase modifies the cytosine residue (C5) of the sequence AGCT.
New England Biolabs, Inc. MscI – 1250 units
A restriction endonuclease that recognizes the sequence TGG_^CCA.